Summary of Study ST000119
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000106. The data can be accessed directly via it's Project DOI: 10.21228/M8RC75 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000119 |
Study Title | Metabolomics analysis of multiple metabolic functions in the YjgF/YER057c/UK114 (Rid) protein family LCMS |
Study Type | wildtype vs knock-out |
Study Summary | Metabolomics analysis of wild-type S. enterica, single RidA knock-out and triple Rid (ridA Rid2 Rid7) knock-out S.enterica cells |
Institute | University of California, Davis |
Department | Genome and Biomedical Sciences Facility |
Laboratory | Fiehn Laboratory |
Last Name | ElBadawi-Sidhu |
First Name | Mona |
Address | 451 Health Sciences Drive, Davis, California 95616, USA |
mmelbadawi@ucdavis.edu | |
Submit Date | 2014-11-06 |
Num Groups | 4 |
Total Subjects | 6 |
Study Comments | Samples were measured in poitive and negative ionization mode. MS/MS files contain product ion scans (MS/MS) spectra for compound identification. |
Raw Data Available | No |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2014-11-06 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000200 | AN000201 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1290 | Agilent 1290 |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak area |
MS:
MS ID: | MS000163 |
Analysis ID: | AN000200 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3000 |
Collision Energy: | Ramped 0.02eV/m/z + 10 eV |
Collision Gas: | Nitrogen |
Dry Gas Flow: | Nebulizer: 10 L/min Sheath: 9 L/min |
Dry Gas Temp: | Nebulizer and Sheath: 350 |
Fragment Voltage: | 125 |
Ionization: | Positive |
Precursor Type: | Intact Molecule |
Reagent Gas: | Nitrogen |
Dataformat: | .d |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Resolution Setting: | Extended Dynamic Range |
Scan Range Moverz: | 100 - 1700 m/z |
Scanning Cycle: | 2 |
MS ID: | MS000164 |
Analysis ID: | AN000201 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Capillary Voltage: | 3000 |
Collision Energy: | Ramped 0.02eV/m/z + 10 eV |
Collision Gas: | Nitrogen |
Dry Gas Flow: | Nebulizer: 10 L/min Sheath: 9 L/min |
Dry Gas Temp: | Nebulizer and Sheath: 350 |
Fragment Voltage: | 125 |
Ionization: | Negative |
Precursor Type: | Intact Molecule |
Reagent Gas: | Nitrogen |
Dataformat: | .d |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Resolution Setting: | Extended Dynamic Range |
Scan Range Moverz: | 100 - 1700 m/z |
Scanning Cycle: | 2 |